Liver-kidney Microfluidic Bioreactor for Cell Coculture in Drug Studies
نویسندگان
چکیده
A microfluidic bioreactor for cell co-culture has been developed to study the cytotoxicity of xenobiotics in multiorgan interaction models. In this paper, we investigate the drug metabolism of ifosfamide, an anticancer drug, in liver and its impact on kidney. HepG2/C3A (liver) and MDCK (kidney) cells are cultured in two separate PDMS chambers connected by a perfusion loop mimicking blood flow. We show that the metabolism of Ifosfamide in liver induces a significant effect on the MDCK cell proliferation in the bioreactor on contrary to monoculture controls.
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